Profluent is seeking to solve the problem of developing and validating novel, functional proteins to revolutionize biomedicine by evaluating and optimizing methods for the delivery of novel gene editing systems in therapeutically relevant cell types.
Requirements
- Extensive hands-on experience with molecular cloning and mammalian cell culture.
- Experience with molecular or cell-based assay development and execution, including qPCR/dPCR, NGS, and/or flow cytometry techniques
- Hands-on experience and/or a deep understanding of key concepts and challenges related to genetic medicine, gene editing systems, DNA repair, etc.
- Experience with process automation and scaling to develop high throughput workflows, ideally in a biotechnology/industry research setting.
- Experience or high-level familiarity with methods for ex vivo and in vivo delivery of genome editing systems
Responsibilities
- Design, execute, and automate robust molecular and/or cell-based workflows for evaluation of delivery modalities for our novel gene editing tools.
- Execute assays to measure on- and off-target activity of novel gene editing systems using a combination of targeted and genome-wide analytical methods.
- NGS library preparation, purification, quantitation, and final quality evaluation.
- Analyze data, interpret results, and present findings to team members to arrive at critical go/no-go decisions.
Other
- BS or MS in molecular biology, biochemistry, or a related field.
- 0-5 years of academic or biotechnology industry research experience.
- Applicants must have ongoing work authorization in the United States that does not require employer sponsorship.
- Legal authorization to work in the United States is required.
- Commitment to physical and mental well-being
