Biohub is seeking to cure or prevent disease through its 501c3 medical research organization, with the support of the Chan Zuckerberg Initiative, by harnessing the human immune system for early disease detection and prevention.
Requirements
- Background in immunology with hands-on experience in immunological assays (e.g., ELISA, cytokine measurements, immune cell functional assays.)
- 1 year of experience with mammalian cell culture, including primary immune cells (e.g., T cells, B cells, monocytes, dendritic cells.)
- Flow cytometry and cell sorting skills, including panel design, instrument operation, compensation, and data analysis (including using FlowJo.)
- Experience with in vitro cell stimulation assays and functional readouts.
- Basic programming skills (Python and/or R) for data processing and visualization.
- Familiarity with additional flow cytometry techniques (e.g., imaging flow cytometry.)
- Knowledge of cell-based screening platforms and plate-based assay formats.
Responsibilities
- Maintain and operate cell sorter and spectral flow cytometers.
- Manage human samples; coordinate sample processing and experimental workflows with collaborating investigators.
- Design and execute complex spectral flow cytometry experiments, including multi-parameter panel optimization and data acquisition.
- Set up and optimize in vitro cell stimulation assays and functional immunological readouts.
- Establish and maintain primary cell culture systems and immortalized cell lines for immunological studies.
- Develop and implement standardized protocols for cell-based assays, including viability assessment, proliferation assays, and cytokine measurements.
- Perform quality control analysis of flow cytometry and immunological assay data to support experimental troubleshooting.
Other
- Bachelor's or Master's degree in immunology, cell biology, molecular biology or a related field.
- Excellent communication skills and demonstrated ability to work collaboratively in team-based research environments.
- Enthusiasm for technology implementation and large-scale project execution and coordination rather than independent hypothesis-driven research.
- Strong organizational skills and attention to detail in executing multi-step experimental workflows.
- Ability to work in a team-oriented, high-throughput environment.
