Xaira Therapeutics needs a Research Associate / Senior Research Associate to support research programs by performing molecular biology techniques, viral vector preparation, and ensuring high fidelity and reproducibility in experiments.
Requirements
- Proficient in library cloning techniques such as vector design, PCR, assembly methods (e.g. Gibson, Golden Gate), and bacterial transformation.
- Hands-on experience with lentiviral production, concentration (e.g. ultracentrifugation or PEG precipitation), titration, and transduction of mammalian cells.
- Familiarity with plasmid design tools (e.g., SnapGene, Benchling) and experience with sequence verification and troubleshooting.
- Strong aseptic technique and experience with mammalian cell culture preferred.
- Experience with next generation sequencing (NGS) as well as high-throughput cloning or automation platforms is a plus.
Responsibilities
- Perform and optimize molecular cloning workflows, including PCR, restriction enzyme digestion, ligation, Golden Gate & Gibson assembly, and transformation.
- Produce, concentrate, and titrate lentiviral vectors for delivery of genetic constructs to mammalian cells.
- Maintain accurate records of constructs, plasmid inventories, viral batches, and QC data.
- Troubleshoot experimental issues independently and suggest improvements to protocols and workflows.
- Contribute to the development of scalable, high-throughput cloning and viral production pipelines.
- Ensure rigorous quality control and documentation of all reagents and processes to enable reproducibility.
- Participate in regular group meetings and present data and progress updates to internal teams.
Other
- B.S./M.S. in Molecular Biology, Cell Biology, Biotechnology, or a related field with 2+ years of laboratory experience.
- Highly organized, with excellent attention to detail and record-keeping in electronic lab notebooks (ELNs).
- Ability to work both independently and collaboratively in a fast-paced, multidisciplinary environment.
- Strong interpersonal and communication skills, with a commitment to transparency and teamwork.
