The Ohio State University's Comprehensive Cancer Center is seeking a Research Senior Technician to support translational research in cancer biology, specifically in the laboratory of Dr. Triparna Sen, with a focus on understanding the mechanisms of initiation, progression, and therapeutic resistance in lung cancer.
Requirements
- Proficiency in cell culture and standard molecular biology techniques
- Experience handling mice, including tumor implantation and drug administration
- Previous lab experience preferably (but not limited to) in cancer biology
- Bacheor’s Degree in Life Sciences or a related field
- Excellent interpersonal skills, problem-solving flexibility, and adaptability able to interface with lab staff
- Adept at planning and prioritizing work to meet commitments with a keen eye for detail
- Action-oriented and eager to take on new opportunities and tough challenges with a sense of urgency, high energy, and enthusiasm
Responsibilities
- Design and execute CRISPR-Cas9-based genetic screens in lung cancer cell models
- Culture and manipulate human lung cancer cell lines and organoids, including transfections and viral transductions
- Perform drug sensitivity assays in vitro and conduct dosing studies in mouse xenograft models
- Establish and maintain PDX and CDX models using immunocompromised mouse strains (NSG, NRG, athymic nude)
- Process human lung tissue specimens for downstream molecular and cellular analyses, including: RNA/protein extraction, flow cytometry and cell sorting, single-cell suspensions for sequencing or culture
- Operate lab equipment including GentleMACS Cell Dissociator, flow cytometers, biosafety cabinets, and centrifuges
- Coordinate mouse studies: monitor health, measure tumor burden, maintain accurate animal records in compliance with IACUC protocols
Other
- Bacheor’s Degree in Life Sciences or a related field
- Excellent interpersonal skills, problem-solving flexibility, and adaptability able to interface with lab staff
- Adept at planning and prioritizing work to meet commitments with a keen eye for detail
- Action-oriented and eager to take on new opportunities and tough challenges with a sense of urgency, high energy, and enthusiasm
- Final candidates are subject to successful completion of a background check. A drug screen or physical may be required during the post offer process
