The Simic group at the Pritzker School of Molecular Engineering is seeking a Research Specialist to help get the lab started and apply cutting-edge approaches to develop next-generation cell therapies, including smart immune cells designed to recognize and kill cancer cells or to treat other challenging diseases such as neuroinflammation and neurodegeneration.
Requirements
- Design and assembly of DNA constructs, including modern molecular cloning techniques like Golden Gate or Gibson Assembly.
- Viral vectors, transposon vectors and/or CRISPR genome integration techniques for genetic engineering.
- Culture of mammalian cells, including tumor cell lines, primary immune cells, such as T cells, and/or stem cells.
- Flow cytometry and cell sorting.
- Animal experiments, including mouse models for cancer, autoimmunity, and/or neurodegeneration, subcutaneous and intravenous injection.
- Immunology assays, such as cytotoxicity, cytokine secretion and general in vitro functional measurements of T cells.
- Fluorescence microscopy and imaging data analysis.
Responsibilities
- Conducting a variety of experimental techniques such as molecular cloning, cell culture (cell lines and primary cells), flow cytometry, and ELISA-like assays.
- Execute cellular assays to collect, analyze, and compile data in support of our research.
- Performs various investigative procedures and operates a variety of instruments and other scientific equipment.
- Helps develop, design, and conduct research projects according to plan.
- Documents research efforts and software adequately for sharing with the PI and lab members.
- Analyzes and maintains data and/or specimens.
- Conducts literature reviews.
Other
- Minimum requirements include a college or university degree in related field.
- Minimum requirements include knowledge and skills developed through < 2 years of work experience in a related job discipline.
- Collaborates with internal and external students, scientists, researchers, programmers, and engineering teams.
- Contributes new ideas that can lead to new experiments and research grant proposals.
- With moderate supervision, manages research-related duties and tasks.
