The Schwörer Lab is interested in understanding the role of the tumor microenvironment in cancer, focusing on cancer-associated fibroblasts. The laboratory combines mouse models and novel culture systems with metabolic flux analysis to investigate how nutrient availability and utilization dictates fibroblast identity and function, and how this contributes to cancer progression.
Requirements
- mammalian cell culture
- 3D organoid culture
- molecular biology techniques (PCR, cloning)
- Western Blot
- RNA isolation and qRT-PCR
- sample preparation for metabolomic analysis
- Experience in handling and working with mice.
Responsibilities
- Design and execute daily laboratory experiments, including mammalian cell culture, 3D organoid culture, molecular biology techniques (PCR, cloning), Western Blot, RNA isolation and qRT-PCR, and sample preparation for metabolomic analysis.
- Work with mouse models, including breeding of mice, weaning pups, genotyping.
- Assist in management of mouse colony.
- Order, receive, label and safely store lab supplies.
- Maintain and renew inventory of laboratory supplies and equipment.
- This job provides technical and administrative support in a laboratory environment performing basic laboratory techniques, research and analysis under direction.
- Provides routine or standardized laboratory duties by collecting data in support of research projects under direct supervision.
Other
- Work on a research project with guidance from the principal investigator and other laboratory members.
- Note keeping and recording experimental data.
- Minimum requirements include vocational training, apprenticeships or the equivalent experience in related field (not typically required to have a four-year degree).
- Minimum requirements include knowledge and skills developed through < 2 years of work experience in a related job discipline.
- Ability to work independently on assigned tasks.
