The Miriam Kim Lab studies methods to manipulate immune cells for treatment of hematologic malignancies. We work at the intersection of hematopoiesis, immunology, cancer biology, stem cell biology, and gene engineering. We are seeking motivated individuals to assist with studies investigating the optimal host environment to support chimeric antigen receptor (CAR) T cell therapy.
Requirements
- Routine laboratory techniques in our lab include: Isolation of primary immune cells from human and mouse samples, including monocytes, T cells, B cells, NK cells, and hematopoietic stem cells.
- Genetic manipulation of immune cells using lentivirus, retrovirus, and/or CRISPR/Cas9-based gene editing.
- Flow cytometry including sample preparation, acquisition and data analysis.
- Mouse husbandry and small animal procedures.
- Laboratory Instrumentation
- Laboratory Research
- Software Program
Responsibilities
- Assists with research studies, experiments and assays, including collection of data, preparation of solutions and setup and maintenance of equipment.
- Performs data entry and maintains data files on research.
- Prepares tissue cultures, reagents, probe tests, etc.
- Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions.
- Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures.
- Assists with general lab maintenance and cleaning.
- Isolation of primary immune cells from human and mouse samples, including monocytes, T cells, B cells, NK cells, and hematopoietic stem cells.
- Genetic manipulation of immune cells using lentivirus, retrovirus, and/or CRISPR/Cas9-based gene editing.
- Flow cytometry including sample preparation, acquisition and data analysis.
- Mouse husbandry and small animal procedures.
Other
- Prior research experience is welcome but not required for this position.
- The ideal candidate will possess intrinsic motivation to conduct innovative and impactful research.
- A growth mindset and ability to learn new concepts and skills is essential.
- The individual will be trained to perform experiments independently and analyze data to produce graphs and figures for publication.
- Career development opportunities may also be provided.
- Bachelor’s degree or combination of education and/or experience may substitute for minimum education.
- The ideal candidate will have up to one year of experience in a laboratory setting.
- Communication
- Detailed Reports
- Working Independently
