The Ohio State University is seeking a Researcher to join the laboratory of Dr. Triparna Sen to support translational research in cancer biology, with a focus on understanding the mechanisms of initiation, progression, and therapeutic resistance in lung cancer.
Requirements
- Master of Science Degree in Life Sciences or a related field
- 5 years of relevant lab experience preferably (but not limited to) in cancer biology
- Proficiency in cell culture and standard molecular biology techniques
- Experience handling mice, including tumor implantation and drug administration
- Proficiency in CRISPR screening platforms
- Experience with drug dosing studies in both in vitro and in vivo settings
- Clinical sample processing, including the development of patient-derived xenograft (PDX) models
Responsibilities
- Design and execute CRISPR-Cas9-based genetic screens in lung cancer cell models
- Culture and manipulate human lung cancer cell lines and organoids, including transfections and viral transductions
- Perform drug sensitivity assays in vitro and conduct dosing studies in mouse xenograft models
- Establish and maintain PDX and CDX models using immunocompromised mouse strains (NSG, NRG, athymic nude)
- Process human lung tissue specimens for downstream molecular and cellular analyses, including: RNA/protein extraction, flow cytometry and cell sorting, single-cell suspensions for sequencing or culture
- Operate lab equipment including GentleMACS Cell Dissociator, flow cytometers, biosafety cabinets, and centrifuges
- Coordinate mouse studies: monitor health, measure tumor burden, maintain accurate animal records in compliance with IACUC protocols
Other
- Excellent interpersonal skills, problem-solving flexibility, and adaptability able to interface with lab staff
- Adept at planning and prioritizing work to meet commitments with a keen eye for detail
- Action-oriented and eager to take on new opportunities and tough challenges with a sense of urgency, high energy, and enthusiasm
- Cover letter (maximum 2 pages) indicating research background, interest in the position, and expected start date
- Curriculum Vitae (CV)
