Generate:Biomedicines is seeking to accelerate drug discovery through advanced imaging technologies, specifically by generating large-scale protein structural datasets using CryoEM.
Requirements
- Recombinant protein expression in E. coli and mammalian cell lines, protein purification (ÄKTA-based), and associated analytical techniques (aSEC, DSF, SDS-PAGE).
- Prior exposure to CryoEM workflows or microscope operation (e.g., SerialEM, Vitrobot, GP2).
- Hands-on experience with liquid handlers (Tecan, Hamilton).
- Experience running binding assays (e.g., Octet or Gator Bio).
Responsibilities
- Drive the advancement of experiments and technologies by performing protein purification, complexation, vitrification, and clipping, ensuring high-quality sample preparation for CryoEM.
- Operate CryoEM microscopes and execute automated data collection workflows with minimal guidance.
- Interpret and troubleshoot experimental data, contributing to planning and prioritization of concurrent CryoEM experiments.
- Apply and refine data analysis methods; determine reliability of results and communicate findings in presentations or team meetings.
- Collaborate with multidisciplinary teams to support high-throughput protein structure determination.
- Document experimental data and report findings in internal databases.
- Contribute to process optimization and implementation of new protocols.
Other
- Bachelors Degree with 4+ or Masters Degree with 2+ years of experience.
- Demonstrated ability to work independently with minimal guidance while contributing to a collaborative research environment.
- Excellent troubleshooting and attention to detail.
- On-site position at our Andover, MA CryoEM lab.
- Commitment to equal employment opportunity regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity or Veteran status.
