Flagship Pioneering is looking for a Senior Research Associate in Protein Sciences to help drive the discovery, optimization, and formulation of novel biologics for novel applications, addressing challenges such as water scarcity.
Requirements
- Proficiency in: Basic molecular biology techniques: cloning, plasmid prep
- Proficiency in: Protein expression in bacteria with research-scale production experience
- Proficiency in: Analytical methods for protein quantification and characterization (e.g. SDS-PAGE, immunoblotting, UV-Vis)
- Proficiency in: Maintaining ELNs and summarizing experimental findings for technical and non-technical audiences.
- Experience with downstream biologic formulation screening
- Prior experience with protein expression in diverse host systems (bacterial, fungal, mammalian)
- A strong understanding of codon optimization strategies and expression vector design.
Responsibilities
- Perform traditional cloning techniques including Gibson Assembly, In-fusion, site-directed mutagenesis, etc.
- Support in silico construct design and vector optimization
- Maintain, transform, and prepare plasmids for various downstream workflows
- Screen and optimize expression conditions (e.g. temperature, induction system, media) in bacterial systems.
- Conduct upstream research-scale fermentation to support recombinant protein production.
- Test and optimize downstream processing methods
- Optimize formulation conditions to enhance stability and activity across various storage and use environments.
Other
- Detail-oriented and highly collaborative scientist
- Entrepreneurial scientist with a passion for innovation and real-world impact.
- Relentless Pioneer capable of charting comprehensive but actionable plans, executing rapidly and iteratively to make progress, and reliable in delivering decisive readouts on time.
- Team player who flourishes in collaborative work environments with other highly-skilled individuals.
- Effective communicator who can convey technical information in a clear, concise, and decisive manner.
