Manifold Bio is seeking to solve the problem of developing novel oligonucleotide therapeutics using a novel in vivo-centric discovery approach, specifically by building oligonucleotide shuttling capabilities and supporting multiple oligo-based disease programs.
Requirements
- Proficient in cell culture practices and methodologies, using a variety of mammalian cells (such as transformed cell lines and patient fibroblasts).
- Nucleic acid methodologies and gene expression analysis including RNA/DNA isolation, RT-PCR, qPCR, agarose gel electrophoresis.
- Protein analysis methodologies including western blot, ELISA, Protein quantification assays.
- Familiarity with laboratory equipment, software and data analysis software.
- Experience with oligonucleotide therapeutics or antisense technologies (siRNA, antisense oligonucleotides).
- Hands-on experience with flow cytometry and microscopy techniques.
Responsibilities
- Prepare and analyze samples from in vitro and in vivo studies using a variety of biochemical and molecular biology techniques such as cell and tissue lysis, nucleic acid extraction, RT-qPCR, and SDS-PAGE.
- Support the development and optimization of assays and experimental methods for the purpose of evaluating oligonucleotide-based therapeutics in disease models.
- Design and perform cell-free and cell-based experiments to develop and implement novel assays and technologies.
- Author and maintain good written records of laboratory procedures and data, study protocols and other scientific documents.
- Present key findings at internal and external scientific meetings.
- Interact and communicate with supervisor, study leads and other team members.
- Support day-to-day laboratory tasks.
Other
- B.S. or M.S. degree in Biology or related field with 5+ years of relevant laboratory research experience, or the equivalent experience obtained through working in a laboratory setting.
- Excellent organization and communication skills and ability to collaborate effectively.
- Ability to work efficiently to meet critical deadlines while maintaining scientific excellence.
- Strong technical expertise combined with independence, adaptability, and a proactive mindset.
- Ability to generate high-quality, reproducible data and take pride in rigorous experimental documentation.
