Colossal Biosciences is seeking to engineer embryonic and pluripotent cell systems for non-model species, specifically marsupial species, to support mammalian de-extinction and conservation programs
Requirements
- Experience working with primary embryonic and iPSC cultures, including maintenance and expansion techniques
- Proficiency in core molecular biology techniques, including qPCR, immunohistochemistry, colony picking, and stem cell passaging workflows
- Familiarity with stem cell reprogramming technologies, particularly iPSC generation and validation
- Experience using electronic lab notebooks (ELNs) and inventory management systems (e.g., Benchling) for data tracking and reagent management
- Experience with RNA extraction, RNA handling, and protein quantification assays (e.g., ELISA and Western blot)
- Experience with cell line derivation, propagation, and transfection methodologies, especially when following established protocols
- Familiarity with sequence analysis software such as SnapGene or Geneious
Responsibilities
- Contribute to the design, development, and execution of novel experiments, techniques, and protocols under the guidance of scientists, with growing autonomy in protocol optimization and troubleshooting
- Perform and support 2D and 3D cell culture of primary, immortalized, ESC, and iPSC lines with high technical accuracy
- Conduct and troubleshoot FACS, Western blotting, qPCR, immunostaining, transfection, and microscopy-based assays
- Support stem cell reprogramming workflows, including those for non-model species, with adaptability and a willingness to learn species-specific protocols
- Maintain and optimize 3D culture systems, including spheroid and organoid models
- Characterize and perform quality control of marsupial stem cell cultures, with training provided where needed
- Document experimental work thoroughly and contribute to reports, presentations, and publications
Other
- Bachelor’s or Master’s degree in biological sciences, molecular and cell biology, bioengineering, biochemistry, developmental biology, or a related field
- Prior experience as a Research Associate or Technician in an academic or biotechnology industry setting is a plus
- Experience with non-model systems or comparative biology is welcomed but not required
- Excellent communication, adaptability, and a strong team-oriented mindset
- Ability to work on-site in Dallas, TX location, with relocation assistance available
